This research was planned to isolate a marine bacterium that can biosynthesize silver nanoparticles to be used for therapeutic applications. The environmentally friendly production of nanoparticles from biological sources is simple, and inexpensive. Silver nanoparticles (AgNPs) are part of a new class of biomaterials that is being created more and more for use in scientific and medical endeavours. Given how important the biological system is, it is essential to have a basic understanding of how inorganic nanoparticles affect cellular development and function. Resazurin, a rapid screening assay, was applied to evaluate the antibacterial effects of the synthesised AgNPs against pathogenic microbes. The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay was utilized to test AgNPs for cell cytotoxicity and anticancer activity. They were also tested for wound healing using the scratch assay on a human epithelial cell line (WISH-CCL-25). Halomonas sp. FSSH, among many isolates successfully biosynthesized AgNPs with interesting properties. The present finding revealed that an extract of Halomonas sp. FSSH could be effectively used as a reducing agent for the green production of AgNPs. These AgNPs are considered efficient antimicrobial, anticancer, and wound-healing agents without cytotoxic effects.