Background: The care of children with cancer is very specialized, and in order to deliver high-quality care, nurses must be educated, skilled, and committed to standard guidelines. Aim: To assess the quality of nursing care provided to children undergoing chemotherapy. Design: A descriptive design was used for this study. Setting: The study was conducted at the Oncology Institution in Sohag City. Sample: A convenient sample of all available nurses (40 nurses) who are working with children undergoing chemotherapy at the Oncology Institution in Sohag City. Tools: Two tools were used for data collection. Tool (I): A personal data sheet. Tool (II): Observational checklist. Results: There was a highly statistical significant statistical positive correlation between the studied nurses assessment of the patient before administration, providing patient/caregiver education, pre-administration considerations, chemotherapy/biotherapy administration, post administration of chemotherapy/biotherapy care and quality of nursing care. Conclusion: On the light of the current study results, 75% of the studied nurses had incompetent level of quality of nursing care provided to children undergoing chemotherapy, while 25% of them had competent level of quality of nursing care provided to children undergoing chemotherapy. Recommendations: Develop training courses regarding chemotherapy administration among pediatric  nurses.