Background: Females with visual impairment require continuous support in their personal hygiene practices. They are particularly at risk due to the challenges on access to health information. Aim: To assess personal hygiene awareness among visually impaired El-Nour school female students. Design: A cross sectional descriptive design. Setting: This study was conducted in El-Nour school at Sohag Governorate. Sample: A cluster random sample of 55 visually impaired adolescent students. Tools: Three tools were used to collect data.  Tool one included two parts; part one: personal characteristics and part two: visually impaired female students' knowledge about personal hygiene. Tool two: Included two parts; part one: The personal hygiene behavior scale and Part two: The Genital Hygiene Behaviors scale. Tool three: Reported practices about hand washing, teeth brushing and perineal care. Results: It was found that 52.7% of the studied students had unsatisfactory knowledge of personal hygiene, 78.2% & 63.6% had negative personal and genital hygiene behavior respectively and 67.3% had unsatisfactory reported practices about hand washing, teeth brushing and perineal care. Also, there were statistically significant differences between visually impaired female students' level of knowledge about personal hygiene and their father's education, mother's education, father's occupation and mother's occupation (P-value = 0.000, 0.001, 0.013 and 0.001) respectively. Conclusion: the studied findings revealed that visually impaired female student had unsatisfactory knowledge about personal hygiene and they had negative personal and genital hygiene behavior. Recommendation: Educational program about personal hygiene habits should be provided for visually impaired female students in the early age.