This research aims to explore the relationship between work-life balance (WLB), career development (CD), organizational support, and gender within private universities in Egypt. While previous studies have mainly focused on the direct association between WLB and CD, this research goes further by investigating the moderating effects of gender and organizational support on this relationship, particularly in the context of private higher-educational institutions in an emerging economy. Despite the well-documented negative consequences of work-life imbalances, such as stress and health issues, which can impede personal relationships and career progress, there has been limited attention given to achieving WLB among academic staff in Egypt. Moreover, research on WLB in developing economies remains scarce, with most studies conducted in developed countries. To address these gaps, the author developed a conceptual model and employed a comprehensive descriptive research design that utilized both qualitative and quantitative methods. The research began with qualitative focus groups and in-depth interviews, followed by the distribution of 283 questionnaires to full-time academic staff members in private universities. The researcher employed quota sampling techniques, ensuring the representation of gender differences within the sample. Data analysis was conducted using SmartPLS software.The findings indicate a significant positive association between WLB and it provides valuable insights for teaching staff members and HR managers in private universities in Egypt, enhancing their understanding of the importance of WLB in fostering academic career development.