Purpose: Female genital candidiasis is a common gynecological complaint. Studies demonstrated its negative impact on sperm motility. In the current study we aimed at determining the effect of vaginally extracted Candida albicans on different semen parameters. Study Design: Vaginal swabs were obtained from 25 females suffering from vaginitis and semen samples from 18 normozoospermia healthy males. Semen samples were inoculated with different concentrations of vaginal isolates of Candida albicans (0 (control), 20000, 60000 and 100000 cfus/0.5ml) for two incubation periods (5 and 15 hours). Semen was tested for sperm motility, vitality, morphology, and fertilizing capacity as well as seminal fructose, seminal level of reactive oxygen species (ROS) and pH. Results: Significant concentration and duration dependent decrease in sperm motility, vitality, normality and sperm fertilizing capacity was observed following incubation with candida. The drop in the percent of normal forms was within the acceptable range for normal semen. Level of ROS decreased significantly only at high candida concentration (100000 cfus/0.5 ml) and duration of incubation (15 hours). Seminal fructose was mainly affected by candida concentration while pH decreased only at low incubation time. However, the observed change in pH was still within the normal semen pH range. Relation between patient's results and types of graft used showed no statistically significant differences between them. Conclusion: Vaginally extracted Candida albicans may have negative impact on different semen parameters affecting mainly sperm motility, vitality as well as sperm fertilizing capacity. To what extent this effect may influence couple fertility needs further studies.