This study aimed to assess the impact of grape seed extract as a crosslinking agent on the micro-tensile bond strength of self-etch and etch-and-rinse adhesives to dentin after 24 hours and
a 3-month aging period.
Eight extracted sound human molars were chosen and subsequently randomized into four primary groups. The teeth were categorized into two equal groups, each including four teeth, based on the surface treatment applied. Group (A1) denotes the control group that does not utilize cross-linking agents. Group (A2) comprises specimens subjected to treatment with Grape Seed extract. Each group will be further separated into two equal subgroups based on the adhesive method employed, with subgroup (B1) indicating the use of a total-etch adhesive system. Subgroup (B2) pertains to the application of a self-etch adhesive method. Subsequently, each group will be separated into two equal classes based on the aging period. T1 denotes aging after 24 hours, whereas T2 signifies aging after 3 months. The results indicated that etch and rinse adhesives exhibit significantly higher bond strength compared to self-etch adhesives. Additionally, GCE markedly enhances the micro-tensile bond strength in self-etch groups relative to their control, whereas in the etch and rinse group, GCE does not significantly improve bond strength compared to the control group. The bonding efficacy of etch-and-rinse adhesives surpasses that of self-adhesives, and pre-treatment of dentin with 6.5% GSE w/v can sustain superior bond strength during storage in artificial saliva.