In Egypt, according to International Diabetes Federation (2021), 10.9 million Egyptians were suffering from diabetes in 2021. Different models are used to induce diabetes in different animals. This study aimed to evaluate the biochemical changes due to induction of diabetes in rats with different methods.
Methods: male albino rats were used in this study. First group is the control group was injected with equivalent amount of 0.9% saline ip to that used for alloxan and STZ preparation, second group was injected with150 mg alloxan/ kg b.wt, third group injected with Streptozotocin (STZ) 60mg/kg b.wt. The fourth group drank high fructose (HF, 20% solution) for 2 months and the fifth group fed high fat diet (HDF, 30 %) for 2 months.
Results: Both alloxan and STZ induced T1DM which was characterized by very high elevation in the blood glucose (405.20±20.28and 424.40±10.31mg/dl) in both groups respectively vs control group (86.70±1.45mg/dl). Also, high decreases of insulin level were recorded in the same groups. HF and HFD groups revealed the presence of slightly hyperglycemia in these rats. Both blood glucose (104.30±3.28 and 104.20±3.48mg/dl) respectively and insulin levels increased in both groups. Many changes of both liver and kidney functions tests altered. All groups had increased oxidative stress marker (MDA) which was associated with decreased antioxidants markers.
Conclusion: the used models for diabetes induction can be used for assessment the diabetes complications and treatment trials.
Key words: diabetes, streptozotocin, alloxan, HF, HFD, biochemical parameters and oxidative stress.