Hepatitis C virus (HCV) infection is a major global disease that frequently causes chronicity and has the potential to cause liver failure and it is widespread in Egypt. Direct acting antivirals (DAAs) are a revolution in HCV treatment. Sofosbuvir (Sovaldi) is pan genotypic while, Daclatasvir has a wide range of HCV genotypes A correlation analysis is the study of how variables are correlated to each other. The current study aimed to explore the potential correlation between quantitatively detected virus C load using quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR) assay and some immunological, biochemical and hematological biomarkers. Results from 600 HCV-positive people ranged between 25 and 55 years old were analyzed using the Prism, a computer program (Graph Pad software. Version 5, San Diego, CA), and concluded that, there were a significant relation between HCV viral load and immuno-biological biomarkers. Also, the present study referred to the efficacy of Sofosbuvir/Daclatasvir combined therapy where 100% Sustained Virological Response (SVR) in all patients.