Diabetes is a major health problem characterized by inadequate hyperglycemia due to relative or absolute insulin deficiency at the cellular level. Dinitrophenol causes weight loss by uncoupling oxidative phosphorylation. While caffeine use on a regular basis is linked to a decreased risk of diabetes and Parkinson's disease. Dinitrophenol and caffeine decrease oxidative stress. Diabetes leads to increased oxidative stress so they can decrease blood glucose. In this research, 70 male albino rats were selected, with body weight ranging between 100-160 g, and divided into 7 groups, a control group, a dinitrophenol group, a caffeine group, and d diabetic group. Rats were given only one dosage of alloxan by intraperitoneal injection. Rats with diabetes were separated into the diabetic group, diabetic-dinitrophenol, diabetic-caffeine, and diabetic-dinitrophenol-caffeine treated groups. After 8 weeks, we measured glucose, insulin, lipid indicators, antioxidants, and oxidative stress markers. The diabetic group showed a very high elevation in glucose levels compared to the control group. The diabetic-dinitrophenol-caffeine group showed more reduction in blood glucose levels. All treated groups showed an increase in insulin levels. Lipid profiles showed variations in different groups. All treated groups had increased oxidative stress markers associated with increased antioxidant markers.
Keywords: Insulin, Hyperglycemia, Antioxidants, oxidative stress, lipid indicators