low-cost bioactive glass-ceramic, akermanite (Ca2MgSi2O7) with and without Cu2O was synthesized. Limestone , Silica sand and Magnesium carbonate served as the parent materials. The prepared materials were investigated using various techniques (DTA, FTIR, XRD, SEM). Glass-ceramic samples were examined for bioactivity, degradation, and ion release in simulated physiological fluids (SBF). Additionally, the antibacterial impact was examined using Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Moreover, the cytotoxicity of Wi-38 cells lines, which are typical fibroblasts, was examined. According to DTA, the presence of Cu2O did not significantly raise the temperature at which glass crystallizes. The crystallization of the glasses into cuspidine, akermanite and diopside was strong at 1000 ˚C. In a glassy matrix, nanosized clusters and submicron crystals can be seen in the microstructure. After immersion in SBF, all samples included nano and submicron hydroxyapatite particles. Bioactivity showed that all samples could form a hydroxyapatite layer after 2 or 4 weeks in SBF. The antibacterial results showed that all of the samples were antibacterial against the germs that were tested. Performing an MTT assay on samples to determine the cytotoxicity, all of the samples showed excellent cytocompatibility and cell proliferation and could be used safely in biomedical applications.