Urinary tract infection is considered a serious public health problem. One hundred and fifty patients were included in the present study and suspected to have community-acquired urinary tract infections depending on crucial indicators. Out of 150 urine cultures, 69.3% were confirmed as urinary tract infection with significant growth and 30.7% was sterile pyuria. Community-acquired urinary tract infections showed high incidence in females (44.1%), followed by kids (32.3%; 19.6% girls and 12.7% boys), and males (23.5%) with differences regarding age groups. Gram staining indicated that 63.2% of total isolated uropathogens were gram-negative isolates, and 36.7% were gram-positive isolates. The identification of 106 bacterial isolates led to the presence of 16 distinct species belonging to 12 genera including Escherichia coli (40.56%), Enterococcus spp. (18.86%), Streptococcus spp. (10.37%), Klebsiella spp. (8.49%), Citrobacter koseri (6.60%), Corynebacterium urealyticum (4.71%), Proteus mirabilis (2.83%), Enterobacter intermedius (1.88%), Pseudomonas aeruginosa (1.88%), Staphylococcus saprophyticus (1.88%), Serratia fonticola (0.94%) and Bacillus cereus (0.94%). Phenotypic detection of ESBL revealed that 35.8% of total isolated gram-negative uropathogens were ESBL-producers. Escherichia coli comprised a serious threat as the most dominant ESBL-producing organism representing 66.7% of total ESBL-producing bacteria detected followed by Klebsiella pneumonia (12.5%), Citrobacter koseri (8.3%), Serratia fonticola (4.1%), Proteus mirabilis (4.1%), and Pseudomonas aeruginosa (4.1%).