Zearalenone is a toxic mycotoxin that is frequently present in foods and feeds. The accumulation of zearalenone in the kidney caused proteinuria and glomerular distortion. The main biological and pharmacological component of honeybee venom is called melittin. This study aimed to examine the therapeutic effects of melittin against rat renal failure induced by zearalenone. Three groups of animals (N = 10) have been generated: control, Zearalenone group, and Zearalenone + Melittin group. Zearalenone was administered orally to rats at a dose of 2.7 mg/kg body weight whereas melittin was administered at a dose of 40 µg/kg body weight. Three groups' values of biochemical alterations were recorded, and statistical analysis was done. Zearalenone reduced kidney weight and increased renal biomarker levels. Renal biomarkers were reduced by melittin, however, all changed biomarkers and renal weight were restored. Biochemical abnormalities were confirmed by histopathological changes. Melittin, therefore, had therapeutic effects that were adaptable to renal dysfunction and damage induced by zearalenone.