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297764

Evaluation of the Antibacterial Activity of Chrysomya albiceps Larval Extract and its Synergistic Effects with Antibiotics

Article

Last updated: 29 Dec 2024

Subjects

-

Tags

Zoology

Abstract

Antimicrobial resistance is currently a significant global problem. The medical field is currently suffering from a shortage of effective treatments to prevent infections since the emergence rate of multidrug resistance has exceeded the rate of discovery and creation of new effective drugs. This study evaluates the antibacterial activity of the excretions/secretions of Egyptian calliphorid larvae, Chrysomya albiceps (C. albiceps), in vitro on the development of five pathogenic bacterial species (methicillin-sensitive Staphylococcus aureus ATCC 29213, methicillin-resistant Staphylococcus aureus ATCC BAA-1680, Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922 and Salmonella typhi ATCC 19430) via different assays. Resazurin-based 25turbidimetric assay (RTBA) proved that C. albiceps larval exosecretions (ES) was effective against all examined bacterial species, which also determined the minimum inhibitory concentration (MIC) for each bacterium. By using a colony-forming unit (CFU) assay, MICs of C. albiceps ES exhibited bacteriostatic activity against all tested bacteria. All the bacterial models could not recover after 24 hrs of the incubation period. However, high concentration (200 mg/ml) showed bactericidal activity towards P. aeruginosa. The potency of C. albiceps extract toward gram-negative bacteria has been proven by agar disc diffusion assay which failed to show any antibacterial efficiency of ES toward gram-positive bacteria. Synergism between larval ES and commercial antibiotics against all bacterial species was observed. The present data is promising and using these combinations can lead to a reduction in the minimum dose required for effective antimicrobial effects which is important because it may decrease both the risk of side effects and the costs of treatment of infectious diseases.

DOI

10.21608/sjsci.2023.195516.1060

Keywords

Antibacterial activity, MRSA, MSSA, Crude extract, Chrysomya albiceps

Authors

First Name

Azza

Last Name

Khdre

MiddleName

M.

Affiliation

Zoology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt.

Email

azzakhdre@yahoo.com

City

Sohag

Orcid

-

First Name

Tarek

Last Name

Ismail

MiddleName

G.

Affiliation

Zoology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt.

Email

t_gad_2000@science.sohag.edu.eg

City

-

Orcid

0000-0002-4361-5588

First Name

Islam

Last Name

Zakaria

MiddleName

M.

Affiliation

Bacteriology Department, Animal Health Research Institute, Agriculture Research Center, Giza, Egypt

Email

islamelshereef25@gmail.com

City

-

Orcid

-

First Name

Gehad

Last Name

Abdelnasser

MiddleName

-

Affiliation

Zoology Department, Faculty of Science, Sohag University, 82524 Sohag, Egypt., Microbiology department, Animal Health Research Institute (AHRI), Agricultural Research Center (ARC), Sohag City, Egypt.

Email

hamzag20130@gmail.com

City

-

Orcid

-

Volume

8

Article Issue

3

Related Issue

41127

Issue Date

2023-09-01

Receive Date

2023-02-22

Publish Date

2023-09-01

Page Start

259

Page End

269

Print ISSN

2357-0938

Online ISSN

2974-4296

Link

https://sjsci.journals.ekb.eg/article_297764.html

Detail API

https://sjsci.journals.ekb.eg/service?article_code=297764

Order

297,764

Type

Regular Articles

Type Code

2,359

Publication Type

Journal

Publication Title

Sohag Journal of Sciences

Publication Link

https://sjsci.journals.ekb.eg/

MainTitle

Evaluation of the Antibacterial Activity of Chrysomya albiceps Larval Extract and its Synergistic Effects with Antibiotics

Details

Type

Article

Created At

29 Dec 2024