In this study, we evaluated the bacterial infections in 120 samples of each urine and blood of chronic kidney disease patients collected from Sohag University Hospital and Sohag Oncology Cancer Centerat Sohag governorate, Egypt. Twenty-two pathogenic bacterial species belonging to 13 genera were isolated and identified from urine and blood samples examined. Twelve of the isolated bacterial species were Gam-positive bacteria and the other nine species were Gram-negative, in addition to one Gram-variable species. Urine bacterial infection (22 species, 13 genera &35836CFU) is higher than blood (10 species, 8 genera &2808CFU). Urine bacterial infection with Gram-negative species was represented by 17007CFUmatching (47.46%) the total count, Gram-positive bacteria represented by 18829CFU(42.82%) and Gram-variable (Lysinibacillus pakistanensisOQ299572) represented by 3484CFU, accounting for (9.78%)of the total count. On the other hand, in blood Gram-negative bacteria was represented by 1676 CFU(59.69%) whereas, Gram-positive bacteria was represented by 850 CFU (30.27%). Gram-variable species (Lysinibacillus pakistanensis OQ299572) represented by 282 CFU as 10.04% of total count.To the best of our knowledge, this is the first report of isolation of Lysinibacillus pakistanensis OQ299572 from human clinical specimens in the world.