Background: Intestinal obstruction in children is a partial or complete blockage that prevents food or liquid from passing through intestine. Aim of the study: Assess knowledge and practices of pediatric nurses about pre and postoperative care of Intestinal obstruction. Design: A descriptive correlational research design was applied on all available nurses (90 nurse) who are working in neonatal intensive care unit, pediatric intensive care unit and surgery department. Tools of Data Collection: A structured interview questionnaire sheet and observational checklists. Results: All studied nurses had unsatisfactory knowledge about intestinal obstruction in children and the majority of them had incompetent practice. Also, there was no statistical significant difference between total nurses' knowledge and their total practice. Conclusion: the study concluded that all studied nurses had unsatisfactory level of knowledge and the majority of them had incompetent level of practice about pre and postoperative care of intestinal obstruction in children with was no statistical significant correlation between total nurses' knowledge and their total practice. Recommendation: Training educational programs and workshops regarding pre and postoperative care of intestinal obstruction should be provided.