Background: Human resources management provide great contributions to create organization vision and improve organization efficiency and productivity. In current century, human resources management has a strategic importance for the competitive power of the organizations. Talented workers are the most important and the greatest value-added item and the provider of competitive advantage in today and in the future. The present study aimed to assess the effect of human resources management training program for nurse managers at Suez Canal University hospitals Subjects and methods: A quasi-experimental design was used to conduct the present study. A random sample (64 nurse managers) divided into two groups: Study and Control group. Data were collected using two tools namely; Human resources management knowledge assessment questionnaire and Human Resource Management practices rating scale. Results: The mean percentage of nurse managers' level of knowledge regarding their human resource management practices post program were 98. 53 % for study group while 29.76% for control group. The total mean percent of human resources management practices post program for the study group of nurse managers was 78.17% while in control group was 43.8% Conclusion: There were significant differences between study and control groups of nurse managers post and follow up program regarding their human resource management practices. It recommended with establishment human resources department instead of staff development department that focuses on implementing all human resources management practices not just the training.