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380136

AGGRESSIVENESS VARIATIONS AMONG ISOLATES OF FUSARIUM OXYSPOIUM F.SP. LYCOPERSICI BASED ON THE PATHOGENICITY TEST, HOST DEFENSE RESPONSE, AND ISOLATES GENETIC VARIABILITY

Article

Last updated: 05 Jan 2025

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Tags

Plant Protection and Phytopathology

Abstract

Fusarium oxysporum f.sp. lycopersici (FOL) is a soilborne fungus and the causal agent of vascular wilt disease in tomato and results in severe yield losses, challenging tomato production in Egypt and worldwide.  As a key step towards controlling this serious disease, this study investigates the aggressiveness of local FOL isolates, and their effects on the plant defense-related enzymes and gene expression, and to estimate the genetic variation among the isolates using RAPD-PCR in relation to their aggressiveness. Nine FOL isolates (Fo1 – Fo9) were isolated from diseased tomato field plants. Tomato (Lycopersicon esculentum Mill) cultivar ‘loggain' was used to study the pathogenicity of the nine isolates and their effects on two defense-related enzymes: (1) Peroxidase (POD), (2) Polyphenol oxidase (PPO), and the gene expression of the two tested enzymes in the host was quantified using Real-time PCR. Three isolates showed different levels of aggressiveness on the tested cultivar (High: Fo5, followed by Fo3 and Fo1; Moderate: Fo8 and Fo7; Weak: Fo9 and Fo4). The highest enzyme activity and gene expression of PPO and POD were induced by isolates Fo5 and Fo3, followed by Fo1, Fo8, Fo7, and Fo2, because they caused moderate responses, while Isolates Fo9 and Fo4 isolates induced low enzyme activity and gene expression in the host. A high similarity (78.4%) was observed between Fo9 and Fo4 (included in subgroup 2 in the phylogenetic tree), followed by Fo2 and Fo9 with a similarity of 76.9%, while the similarity between Fo7 and Fo8 was 75.5% (included in group 1 in the phylogenetic tree). The lowest similarity (47.3%) was observed between Fo6 and Fo3. These findings showed differences in pathogenicity among the isolates and suggest a connection between the genetic variability and the aggressiveness of the various isolates.

DOI

10.21608/jaesj.2024.318115.1196

Keywords

Defense-related enzymes. Pathogen, aggressiveness, phylogenetic, Gene expression

Authors

First Name

Sawsan

Last Name

El Shamy

MiddleName

-

Affiliation

Department of plant pathology, Faculty of Agriculture, Damanhur University, El-Beheira, Egypt

Email

sawsan.elshamy@agr.dmu.edu.eg

City

-

Orcid

-

First Name

Ibrahim

Last Name

Adss

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture, Damanhur University, El-Beheira, Egypt

Email

adssibrahim@agr.dmu.edu.eg

City

Damanhour

Orcid

-

First Name

Alsayed

Last Name

Mashaheet

MiddleName

M

Affiliation

Department of plant pathology, Faculty of Agriculture, Damanhur University, El-Beheira, Egypt

Email

a.mashaheet@dmu.edu.eg

City

-

Orcid

-

Volume

23

Article Issue

2

Related Issue

49084

Issue Date

2024-08-01

Receive Date

2024-09-04

Publish Date

2024-09-16

Page Start

221

Page End

249

Print ISSN

1687-1464

Online ISSN

2735-5098

Article File

JAESJ_Volume 23_Issue 2_Pages 221-249.pdf

PDF . 758.6kB

Link

https://jaesj.journals.ekb.eg/article_380136.html

Detail API

https://jaesj.journals.ekb.eg/service?article_code=380136

Order

380,136

Type

Full research articles

Type Code

2,324

Publication Type

Journal

Publication Title

Journal of Agricultural and Environmental Sciences

Publication Link

https://jaesj.journals.ekb.eg/

MainTitle

AGGRESSIVENESS VARIATIONS AMONG ISOLATES OF FUSARIUM OXYSPOIUM F.SP. LYCOPERSICI BASED ON THE PATHOGENICITY TEST, HOST DEFENSE RESPONSE, AND ISOLATES GENETIC VARIABILITY

Details

Type

Article

Created At

29 Dec 2024