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391908

Characterization of Brucella organisms isolated from camels and some associated risk factors of brucellosis in Aswan governorate, Egypt

Article

Last updated: 29 Dec 2024

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Subjects

-

Tags

Microbiology.

Abstract

In this study, isolation, identification and molecular characterization of Brucella isolates detected from camels from different localities in Aswan governorate were performed. Ten Brucella isolates were recovered in this study, these included two from lymph nodes, three from udder tissues, one from testicular tissues, two from hygromas and two from milk secretions of the udder of slaughtered camels. Typing of these isolates revealed six Brucella abortus isolates and four Brucella melitensis. Brucella abortus was recovered from three udder tissues, one testicular tissue and two hygromas while Brucella melitensis was detected from two lymph nodes and two milk samples collected from the udder of slaughtered she camels. In the current study, the real-time PCR assured the identification of Brucella DNAs in the 10 bacteriologically identified Brucella isolates as six B. abortus and four B. melitensis. PCR results in this research confirmed the results of bacteriological examinations and cleared the power of PCR testing at detection and characterization of Brucellae. Therefore, it can be applied to complement the results gained from phenotypic tests. Different risk factors of Brucella infection in camels were investigated and discussed.

DOI

10.21608/aujst.2024.325170.1129

Keywords

BRUCELLAE, camel, PCR, Brucella DNAs

Authors

First Name

Hosein

Last Name

Hosein

MiddleName

I.

Affiliation

Department of veterinary medicine Faculty of veterinary medicine Beni suef university Beni suef Egypt

Email

hoseinabdalaal2014@yahoo.com

City

Beni suef

Orcid

-

First Name

Aml

Last Name

Abdel-Ra’ouf

MiddleName

M.

Affiliation

Department of Animal Medicine, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt.

Email

aml.raouf@yahoo.com

City

Aswan

Orcid

-

First Name

Mohamed

Last Name

Karmi

MiddleName

-

Affiliation

Department of food hygiene, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt.

Email

karmy99@yahoo.com

City

Aswan

Orcid

-

First Name

Sherin

Last Name

Rouby

MiddleName

R.

Affiliation

Department of veterinary medicine Faculty of veterinary medicine Beni suef university Beni suef Egypt

Email

shereen.rouby@vet.bsu.edu.eg

City

Beni suef

Orcid

-

First Name

Mai

Last Name

boghdadi

MiddleName

A. S.

Affiliation

Animal health researches institute, Aswan laboratory

Email

dr.maisalem87@gmail.com

City

-

Orcid

-

First Name

El Toukhy

Last Name

I.

MiddleName

E.

Affiliation

Animal health researches institute, Aswan laboratory

Email

eltokhy_essam@yahoo.com

City

Aswan

Orcid

-

First Name

Bahaa

Last Name

Madkour

MiddleName

S.

Affiliation

Department of Animal Medicine, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt.

Email

bahaaeldeenmadkour@gmail.com

City

Aswan

Orcid

-

Volume

4

Article Issue

4

Related Issue

51586

Issue Date

2024-12-01

Receive Date

2024-10-01

Publish Date

2024-12-01

Page Start

94

Page End

105

Print ISSN

2735-3087

Online ISSN

2735-3095

Article File

AUJST_Volume 4_Issue 4_Pages 94-105.pdf

PDF . 545.5kB

Link

https://aujst.journals.ekb.eg/article_391908.html

Detail API

https://aujst.journals.ekb.eg/service?article_code=391908

Order

391,908

Type

Original papers

Type Code

2,312

Publication Type

Journal

Publication Title

Aswan University Journal of Sciences and Technology

Publication Link

https://aujst.journals.ekb.eg/

MainTitle

Characterization of Brucella organisms isolated from camels and some associated risk factors of brucellosis in Aswan governorate, Egypt

Details

Type

Article

Created At

29 Dec 2024