A total of 1782 camels were employed for serological diagnosis of Brucella infection in different localities at Aswan governorate. Blood sera were screened serially using modified Roe Bengal plate test (mRBPT 75), and positive samples were assured by complement fixation test (CFT). Positive results were considered in samples that gave positive results in both screening and confirmatory tests. Blood sera of 35 camel sera including 27 samples positive in both modified RBPT (mRBT) and complement fixation test (CFT) were subjected to modified rose Bengal test (mRBT), standard Rose Bengal Test 25 μ seruml, (sRBT), Tube agglutination test (TAT), Complement fixation test (CFT) and Real time PCR test (RT-PCR) as parallel testing. The real-time PCR targeting the DNA of Brucella was used for revelation of Brucella DNA in camel serum. Results of the RT-PCR on camel's blood sera revealed the presence of Brucella DNA in blood sera of 28 camels including (19 Brucella abortus DNA and 11 Brucella melitensis DNA) as 2 camel's sera showed mixed infection. Results of different serological assays in this study by parallel testing revealed 29 (82.86%), 22 (62.86 %), 27 (77.14 %), 30 (85.71%),using (mRBPT) 75 μ serum, s (RBT) 25 μ serum, CFT, and (TAT). mRBT revealed 100 %, 85.71%, 96.55% and 100% relative sensitivity, relative specificity, positive predictive value and negative predictive value for mRBT respectively. sRBT revealed 78.6%, 100%, 100 % and 53. 8% respectively. TAT showed 100%, 71.43%, 93.33%, and 100% respectively. CFT revealed 96.43%, 100%, 100 % and 87.50 % respectively.