The current study was carried out in Agricultural Research Station of Al-Marashda, Qena Governorate, A.R.C., Egypt, during seasons of 2023 and 2024. The aim of study was to explore the influences of different pre-germination treatments on seed germination and seedling growth characteristics of Cordia myxa L. The experiment was arranged in a completely randomized block design with 9 treatments, three replications for each treatment. The pre-germination treatments were control (un-treated seeds); soaking in tap-water for 24 and 48 h; soaking seeds in previously boiled water for 24 h at ambient temperature; soaking in GA3 2000, 4000 and 6000 ppm for 24 h; mechanical scarification (abrasion seed coat with sand paper) and immersion seeds in concentrated sulphuric acid (H2SO4) for 5 min. The obtained results showed that the highest germination (G%, DGS, MGR and VI) and seedling growth characteristics (stem length, root length, stem diameter, number of leaves and fresh and dry weights of shoots and roots) of C. myxa was attained when soaking seeds in GA3 at rates of 4000, 2000 and 6000 ppm for 24 h, respectively. The minimum values were shown in untreated seeds. The present investigation concluded the possibility of the Cordia trees species to be responded to breaking seed dormancy using 4000 ppm GA3.