Acrylamide is an organic highly toxic compound that induces neurotoxic influences, it's extensively used in the industrial fields, especially in food products manufacturing. It is necessary to find natural compounds that can counteract their neurotoxic patterns. This study was carried out to highlight the possible neuroprotective mechanisms of garlic oil (GO) upon the biological changes induced by Acrylamide in rats. Forty rats were randomly sub-divided into four experimental groups: control, garlic oil, acrylamide, and acrylamide plus garlic oil. After recording the neurological symptoms, rats from each group were decapitated and brain tissues were excised for biochemical, molecular, and histopathological evaluation. In the Acrylamide group, rats showed ataxia and weakness followed by hind limb paralysis. On the genetic level, showing low mRNA and protein expression of the VEGF indicated acrylamide genotoxicity, affecting the brain blood supply which was confirmed histopathologically by congestion, and hemorrhage. The group of acrylamides co-treated with garlic oil, rats showed an interesting improvement in the previously mentioned clinical signs and retrieved mRNA and protein expression of the VEGF. Treatment with GO improved the biochemical and morphological features of the brain. They enhanced our hypothesis about the oral administration of GO which provides protection and healing ability upon acrylamide neurotoxicity via modulations of both antioxidant and oxidative activities.