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374175

Development of polymerase chain reaction coupled with a high-resolution melting technique for detection of Helicobacter pylori clarithromycin resistance

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Last updated: 28 Dec 2024

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Abstract

Helicobacter pylori, a bacterial pathogen that causes severe gastric diseases, has developed antibiotics resistance; mainly against clarithromycin. Consequently, the latest international recommendations have advised clarithromycin susceptibility testing before prescribing the first line of therapy. This resistance is predominantly related to the A2143G point mutation of 23S rRNA. The aim of this study was to develop a highly sensitive and cheap molecular technique to detect H. pylori clarithromycin resistant strains. For that, plasmids of H. pylori 23S rRNA region harboring A2143G point mutation were constructed and used to develop polymerase chain reaction coupled with a high-resolution melting technique (PCR-HRM). Then, this method was applied on 233 gastric H. pylori positive samples. The obtained results showed that the developed PCR-HRM technique allowed specific identification of clarithromycin resistant and heteroresistant H. pylori samples, even if the ratio of resistant/ sensitive samples was low.  In this series, the detected rate of H. pylori clarithromycin resistance was 7.6 %, which concord with the resistance rate determined using molecular sequencing. So, the developed PCR-HRM represents a good tool for detecting resistant and sensitive H. pylori samples and can be used in routine clinical practices. This will help in managing H. pylori infection, increase its eradication rate, and avoid the use of therapeutic protocols that have serious side effects.

DOI

10.21608/nrmj.2024.305189.1652

Keywords

H. pylori, PCR-HRM, 23S rRNA, Clarithromycin resistance, Hetero-resistance

Authors

First Name

Souad

Last Name

Oirdi Zahir

MiddleName

-

Affiliation

URL CNRST N°15 Laboratory of Human Pathology, Biomedicine and Environment, Faculty of Medicine, Pharmacy and dental Medicine of Fez (FMPDF), Sidi Mohammed Ben Abdellah University (USMBA), Fez, Morocco

Email

souad.oirdizahir1@usmba.ac.ma

City

-

Orcid

0009-0008-4101-5599

First Name

Mounia

Last Name

El Khadir

MiddleName

-

Affiliation

The Higher Institute of Nursing Professions and Health Techniques (ISPITS), Taza, Morocco

Email

mounia.elkhadir@usmba.ac.ma

City

-

Orcid

0000-0002-8426-5078

First Name

Dafr-Allah

Last Name

Benajah

MiddleName

-

Affiliation

Department of Hepato-gastroenterology Hassan II University Hospital Center, Fez, Morocco

Email

dafrb@yahoo.fr

City

-

Orcid

0000-0001-6513-1042

First Name

Sidi

Last Name

Adil Ibrahimi

MiddleName

-

Affiliation

Department of Hepato-gastroenterology Hassan II University Hospital Center, Fez, Morocco

Email

-

City

-

Orcid

0000-0002-2873-0588

First Name

Laila

Last Name

Chbani

MiddleName

-

Affiliation

Department of Pathological Anatomy Hassan II University Hospital Center, Fez, Morocco

Email

chbanil@yahoo.fr

City

-

Orcid

0000-0002-9843-4012

First Name

Mohamed

Last Name

El Abkari

MiddleName

-

Affiliation

URL CNRST N°15 Laboratory of Human Pathology, Biomedicine and Environment, Faculty of Medicine, Pharmacy and dental Medicine of Fez (FMPDF), Sidi Mohammed Ben Abdellah University (USMBA), Fez, Morocco

Email

yassine.abkari@gmail.com

City

-

Orcid

0000-0002-7068-6162

First Name

Bahia

Last Name

Bennani

MiddleName

-

Affiliation

Laboratory of Microbiology and Molecular Biology, FMPDF, USMBA, Fez, Morocco

Email

bahia.bennani@usmba.ac.ma

City

-

Orcid

0000-0002-5509-3991

Volume

8

Article Issue

4

Related Issue

49387

Issue Date

2024-07-01

Receive Date

2024-07-05

Publish Date

2024-08-15

Page Start

2,542

Page End

2,554

Print ISSN

2537-0286

Online ISSN

2537-0294

Article File

NRMJ_Volume 8_Issue 4_Pages 2542-2554.pdf

PDF . 509.1kB

Link

https://nrmj.journals.ekb.eg/article_374175.html

Detail API

https://nrmj.journals.ekb.eg/service?article_code=374175

Order

374,175

Type

Original Article

Type Code

2,265

Publication Type

Journal

Publication Title

Novel Research in Microbiology Journal

Publication Link

https://nrmj.journals.ekb.eg/

MainTitle

Development of polymerase chain reaction coupled with a high-resolution melting technique for detection of Helicobacter pylori clarithromycin resistance

Details

Type

Article

Created At

28 Dec 2024