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352759

Use of rpoB gene phylogenetic marker-based distinction of abiotic stress tolerant and plant-growth promoting Bacillus paralicheniformis isolates from their closely related Bacill

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Last updated: 28 Dec 2024

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Abstract

Bacillus paralicheniformis is a new identified species, which was distinguished from Bacillus licheniformis in 2015 through extensive phylogenomic and phylogenetic analyses. In this context, this study aimed to achieve a clear identification of the active plant-growth promoting rhizobacteria (PGPR) B. paralicheniformis isolates among the closely related B. licheniformis through molecular typing, helping for the development of clearly-identified PGPR isolates to be used as biofertilizers. A total of 15 rhizobacteria were isolated from the olive rhizosphere soils. These bacterial isolates exhibited various proprieties in terms of abiotic stress tolerance, biofilm formation under stress conditions, and enzyme activities (i.e., lipases, cellulases, and proteases). In addition, several PGP traits such as phosphate solubilization and the production of siderophores and indol-3 acetic acid were also observed. Molecular identification through 16S rRNA sequencing initially identified all the isolates as Bacillus spp. The multi-locus sequence typing (MLST) scheme, using six housekeeping genes (adk, ccpA, recF, rpoB, sucC, and spo0A) had unveiled distinct allelic profiles resulting in 13 unique sequence types (ST). Notably, a comprehensive analysis indicated no exact allele matches existed between the examined isolates and those documented in the PubMLST database. Among the six housekeeping genes, we noticed that rpoB gene (RNA polymerase, subunit beta) had multiple polymorphic sites that were bordered by conserved sequences. Furthermore, the phylogenetic analysis based on rpoB had identified clusters indicating distinct phylogenetic relationships between B. paralicheniformis and B. licheniformis, and successfully differentiated the two species from a pool of 90 strains. The rpoB partial sequence proved to be effective for accurate species discrimination.

DOI

10.21608/nrmj.2024.280135.1508

Keywords

Bacillus paralicheniformis, Bacillus licheniformis, rpoB gene, PGPR, MLST

Authors

First Name

Mohammed

Last Name

Ajdig

MiddleName

-

Affiliation

Microbiology and Molecular Biology Team, Center of Plant and Microbial Biotechnology, Biodiversity and Environment, Faculty of Sciences, Mohammed V University, Rabat, Morocco

Email

ajdig988@gmail.com

City

-

Orcid

0000-0003-4616-2104

First Name

Bahia

Last Name

Rached

MiddleName

-

Affiliation

Genopath Research Center, ERNN, Faculty of Medicine and Pharmacy, University Mohammed V of Rabat, Morocco

Email

bahia.rached@gmail.com

City

-

Orcid

0009-0006-4864-3090

First Name

Ahlam

Last Name

Mbarki

MiddleName

-

Affiliation

Functional Genomic Platform/National Centre for Scientific and Technical Research (CNRST), Rabat, Morocco

Email

ahlammbarki97@gmail.com

City

-

Orcid

0009-0007-3005-6821

First Name

Taha

Last Name

Chouati

MiddleName

-

Affiliation

Laboratory of Chemistry-Physics and Biotechnology of Biomolecules and Materials, Faculty of Science and Technology, Mohammedia, Hassan II University, Casablanca, Morocco

Email

taha.chouati@gmail.com

City

-

Orcid

0000-0002-1871-5624

First Name

Chouhra

Last Name

Talbi

MiddleName

-

Affiliation

Microbiology and Molecular Biology Team, Center of Plant and Microbial Biotechnology, Biodiversity and Environment, Faculty of Sciences, Mohammed V University, Rabat, Morocco

Email

chouhra.talbi33@gmail.com

City

-

Orcid

0000-0003-2639-1401

First Name

Elmostafa

Last Name

El Fahime

MiddleName

-

Affiliation

Laboratory of Chemistry-Physics and Biotechnology of Biomolecules and Materials, Faculty of Science and Technology, Mohammedia, Hassan II University, Casablanca, Morocco

Email

m.elfahime@gmail.com

City

-

Orcid

0000-0001-7812-0641

First Name

Marouane

Last Name

Melloul

MiddleName

-

Affiliation

Functional Genomic Platform/National Centre for Scientific and Technical Research (CNRST), Rabat, Morocco

Email

melloul@cnrst.ma

City

-

Orcid

0000-0002-1821-7537

Volume

8

Article Issue

3

Related Issue

47434

Issue Date

2024-05-01

Receive Date

2024-03-28

Publish Date

2024-05-01

Page Start

2,414

Page End

2,434

Print ISSN

2537-0286

Online ISSN

2537-0294

Article File

NRMJ_Volume 8_Issue 3_Pages 2414-2434.pdf

PDF . 746.1kB

Link

https://nrmj.journals.ekb.eg/article_352759.html

Detail API

https://nrmj.journals.ekb.eg/service?article_code=352759

Order

352,759

Type

Original Article

Type Code

2,265

Publication Type

Journal

Publication Title

Novel Research in Microbiology Journal

Publication Link

https://nrmj.journals.ekb.eg/

MainTitle

Use of rpoB gene phylogenetic marker-based distinction of abiotic stress tolerant and plant-growth promoting Bacillus paralicheniformis isolates from their closely related Bacillus licheniformis

Details

Type

Article

Created At

28 Dec 2024