Date palm (Phoenix dactylifera L.) is one of the strategic plants in Egypt and the Middle East.Date palm (Phoenix dactylifera L.) is a crucial crop in arid and semi-arid regions. Tissue culture, a modern propagation technique, is often hindered by contamination. This study investigated the potential of silver nanoparticles (AgNPs) and silver nitrate (AgNO3) to enhance date palm tissue culture by controlling contamination and promoting growth. AgNPs were synthesized and characterized, exhibiting an absorption peak at 405 nm and a particle size of 43.8 nm. The minimum inhibitory concentration (MIC) of AgNPs and AgNO3 against Bacillus subtilis was determined. AgNPs at 100 mg/L exhibited the highest antimicrobial activity.AgNPs and AgNO3 were applied to date palm immature female inflorescence explants at various concentrations. AgNPs at 50 mg/L significantly increased embryo and shoot formation. Biochemical analysis revealed that AgNO3 at 50 and 100 mg/L elevated total phenol and IAA levels, while AgNPs at 100 mg/L increased gibberellins. Antioxidants were significantly higher in explants treated with 25 mg/L AgNPs.Genetic stability analysis using ISSR-PCR confirmed that the treatments did not induce significant genetic alterations.These findings highlight the potential of AgNPs and AgNO3 as effective agents for improving date palm tissue culture efficiency and quality