Beta
390461

Role of Picloram, PEG and Sugar Alcohol on Somatic Embryogenesis of Date Palm cv. Barhee Immature Female Inflorescence

Article

Last updated: 05 Jan 2025

Subjects

-

Tags

-

Abstract

Among the various explants used to propagate date palm, immature inflorescence has been recently used in a wide range. Somatic embryogenesis approach appears to be a reliable option for mass propagation. However, maturation, germination and plant regeneration from somatic embryos are the main challenges of this approach. This research describes in vitro propagation of date palm ‘Barhee‟ through immature inflorescence. Spiklet explants were removed gently from sterilized spaths and cultured on MS medium supplemented with picloram (1.25, 2.5 and 5mg/l). Within 6 subcultures, the direct somatic embryos and embryonic callus were took place in different treatments at various levels. Embryonic callus were shifted onto maturation medium augmented with sorbitol, mannitol and PEG at 5, 10 and 15g/l to record their impact on embryonic callus growth and somatic embryo formation. Subsequently, somatic embryo clusters formed previously were cultured on germination medium with the same concentrations of osmoticum types to form healthy shoots. Paclobutrazol and sucrose at different levels and combinations were added to rooting medium to improve root system. The highest percentages of direct somatic embryos and embryonic callus were achieved on 5mg/l picloram. The addition of PEG to maturation medium at 10 g/l maximized somatic embryo numbers, while mannitol at 15 g/l encouraged significantly the embryonic callus fresh weight. On germination and multiplication stage, mannitol was found to be more effective in the case of shoot formation. PBZ-enriched medium has the positive effect on root formation. Healthy rooted plantlets were successfully transferred to greenhouse conditions with higher survival percentage.

DOI

10.21608/esjp.2024.390461

Keywords

embryonic callus, embryo maturation & germination, Mannitol, Paclobutrazol, Sorbitol

Authors

First Name

Malhat

Last Name

Mervat

MiddleName

M.

Affiliation

Tissue Culture Laboratory of Agricultural Genetic Engineering Research Institute, Agriculture Research Center, Giza, Egypt.

Email

-

City

-

Orcid

-

First Name

El-Homosany

Last Name

A.

MiddleName

Ahmed

Affiliation

In vitro Storage Plant and Cryopreservation, National Gene Bank, Agricultural Research Center. Giza. Egypt.

Email

-

City

-

Orcid

-

First Name

Mona

Last Name

Bakr

MiddleName

-

Affiliation

The Central Laboratory of Date Palm Researches and Development, Agricultural Research Center, Giza,12619, Egypt.

Email

monabakr32@yahoo.com

City

-

Orcid

-

Volume

4

Article Issue

1

Related Issue

51435

Issue Date

2024-01-01

Receive Date

2024-11-04

Publish Date

2024-01-01

Page Start

1

Page End

19

Online ISSN

2735-5152

Link

https://esjp.journals.ekb.eg/article_390461.html

Detail API

https://esjp.journals.ekb.eg/service?article_code=390461

Order

390,461

Type

Researches

Type Code

2,258

Publication Type

Journal

Publication Title

Egyptian International Journal of Palms

Publication Link

https://esjp.journals.ekb.eg/

MainTitle

Role of Picloram, PEG and Sugar Alcohol on Somatic Embryogenesis of Date Palm cv. Barhee Immature Female Inflorescence

Details

Type

Article

Created At

28 Dec 2024