In this study, 216 isolates—104 Cladosporium, 94 Penicillium, and 18 Talaromyces—were evaluated for lipolytic activity at 10 °C on agar medium. Of the total isolates, 167 (77 Cladosporium, 81 Penicillium, and 9 Talaromyces) yielded positive results, accounting for 77.31 % of total. Of these, 40 isolates (14 Cladosporium, 25 Penicillium, and 1 Talaromyces) demonstrated high lipase activity, 54 moderate (25 Cladosporium, 22 Penicillium, and 7 Talaromyces), and 73 isolates (38 Cladosporium, 34 Penicillium, and 1 Talaromyces) displayed low lipolytic activity. All forty of the isolates from the first screening that had significant lipase activity at 10 °C were selected for the secondary screening under submerged fermentation conditions (SmF). Although in varying amounts, every isolate tested in the submerged fermentation secondary screening experiment was able to produce lipase. At 5 ºC, lipase's specific activity ranged from 6.1 to 353.4 U/mg protein; at 10 ºC, it was 3.83 to 353.34 U/mg; at 15 ºC, it was 6.77 to 463.75 U/mg; and at 20 ºC, it was 4.38 to 485.84 U/mg. Based on sequencing of internal transcribed spacer region (ITS), the two most active strains were identified as Cladosporium cladosporioides AUMC 15908 and Talaromyces pinophilus AUMC 308.