Gallbladder stone (GBS) formation is one of the most prevalent and costly gastrointestinal tract disorders in the world; to find the risk factors of GBS formation; 166 patients were admitted to Sohag University Hospital (63 cases had GBS, while the other 103 had no GBS) were included in the study. Their ages ranged from 15-85 ±3 years; The possible risk factors for GBS formation are family history of GBS, excessive fatty meal intake, higher body weight, higher body height, increased abdominal circumference, higher body mass index (BMI), chronic liver disease (CLD), presence of hepatic focal lesions, splenic enlargement and positive hepatitis C virus antibodies (HCV Abs). Multivariate regression analysis showed that the independent risk factors for GBS formation were positive family history and excessive fatty meal intake; Positive family history of GBS and excessive fatty meal intake were the independent risk factors for GBS formation.