The number of infected cattle with tuberculosis in Egypt is increasing annually, probably as a result of the importation of live animals from countries where bovine tuberculosis is predominant. Therefore, the current study was conducted to investigate the prevalence of tuberculosis in imported beef cattle. During the period from September 2020 to March 2022, 2150 slaughtered beef cattle were examined. Sixty animals (2.78%) of them showed tuberculous lesions grossly. Depending on the gross morphologic lesions, tuberculous cases were classified into generalized tuberculosis (9 cases, 15%) and localized tuberculosis (51, 85%). Localized cases were subdivided into pulmonary tuberculosis (28, 46.66%) and extra-pulmonary tuberculosis (23, 38.33%). Specimens were collected for histopathological examination, granuloma staging, and Ziehl-Neelsen (ZN) staining. Based on the histopathological examination, the granulomas were classified into: Stage I (158, 21.43%), Stage II (187, 25.37%), Stage III (156, 21.16%), and Stage IV (236, 32.02%). In conclusion, our study reveals that imported beef cattle slaughtered in Aswan Governorate have a high incidence of tuberculosis. Accordingly, we recommend strict quarantine measures for imported beef cattle to decrease the possibility of disease transmission.