The present study was conducted during three successive seasons, 2020, 2021, and 2022 on thirty-six uniform 6-year-old Flame seedless grapevines in a Private vineyard in the Gohaina region, Sohag, Egypt. This study examined how grapevines respond physiologically, in terms of growth, yield, and quality, to Hydrogen Cyanamide and seaweed extract (Halamphora coffeaeformis) as dormancy breaking, as well as Nano fertilizers (nano Fe+ Zn), H.coffeaeformis and conventional fertilizers (EDTA Fe + Zn) applied three times at on the same vines at fruit set, berry size 6:8 mm and at veraison stage as single or combined. As a result, the maximum value of growth and yield parameters and chemical characteristics were recorded to combined application of dormix 5% the first week of Jan.× H.coffeaeformis at 1ml/l and (Nano Fe + Zn)1_ppm (as an average of the three studied seasons) aspects than using each material alone. So, it is concluded that the combined foliar application dormix 5% first week of Jan. once and H. coffeaeformis at 1ml/l and (Nano Fe + Zn) 1_ppm applied three times on the same vines at fruit set, berry size 6:8 mm and at veraison stage (Coloring 10 to 15% of the berries) led to clear enhancements in the majority of the tested vegetative and fruiting parameters of Flame seedless grapevines.