Beta
286587

The Possible Toxic Effects of Glutathione Intramuscular Injection on Ovaries of Female Albino Rats (Experimental Study)

Article

Last updated: 05 Jan 2025

Subjects

-

Tags

Healthcare research

Abstract

Background and objective: Glutathione can alter the depigmentation of skin and convert eumelanin to pheomelanin during melanogenesis. As a result, glutathione has become one of the most used skin-whitening agents, and women have become obsessed with it. So, the purpose of this study was to see how intramuscular glutathione injection can affect the ovaries of female albino rats.

Methods: 50 female albino rats were involved. The animals were divided into two groups; A: the control group and B: the glutathione-injected group, which received glutathione intramuscularly three times per week for three consecutive weeks at a dose of 30 mg/day. The injected group was subdivided into four subgroups based on how soon after the last dose the rats were sacrificed (B1: immediately, B2: after 24h, B3: after one week and B4: after two weeks). Ovarian tissue samples were collected from all groups. Each sample was subjected for histopathological examination and estimation of estrogen and progesterone by ELISA. Malondialdehyde (MDA) and reduced glutathione (GSH) levels also were detected.

Results: MDA level was significantly higher in subgroups of group B than group A, while GSH level was significantly reduced. The histopathological examination revealed that glutathione injections led to edematous stroma and luteoma. As regard hormones levels, there was significant increase in all (B) subgroups compared to control group. In this study, ovarian changes caused by the prooxidant behavior of glutathione, were reduced partially after two weeks.

Conclusion: External glutathione may function as a pro-oxidant and have negative effect on the ovaries.

DOI

10.21608/mjmr.2023.181492.1320

Keywords

Estrogen, progesterone, ELISA and MDA

Authors

First Name

Mariam

Last Name

Kamel

MiddleName

Kamal

Affiliation

Department of Forensic medicine and clinical toxicology, Faculty of Medicine, Minya University, Egypt

Email

mariam.kamal@mu.edu.eg

City

-

Orcid

-

First Name

Sahar

Last Name

Habib

MiddleName

Refaat

Affiliation

Department of Forensic medicine and clinical toxicology, Faculty of Medicine, Minya University, Egypt

Email

sahr.habiab@mu.edu.eg

City

Minya

Orcid

-

First Name

Azza

Last Name

Abdel Zaher

MiddleName

Mohamed

Affiliation

Department of Pathology, Faculty of Medicine, Minya University, Egypt

Email

azza.zedan@mu.edu.eg

City

Minya

Orcid

-

First Name

Nada

Last Name

Mohamed Fathi

MiddleName

Ahmed Yousri

Affiliation

Department of Forensic medicine and clinical toxicology, Faculty of Medicine, Minya University, Egypt

Email

nada.ahmed@mu.edu.eg

City

Minya

Orcid

-

Volume

35

Article Issue

1

Related Issue

46232

Issue Date

2024-01-01

Receive Date

2023-01-27

Publish Date

2024-01-01

Page Start

167

Page End

177

Online ISSN

2682-4558

Link

https://mjmr.journals.ekb.eg/article_286587.html

Detail API

https://mjmr.journals.ekb.eg/service?article_code=286587

Order

22

Type

Original Article

Type Code

2,212

Publication Type

Journal

Publication Title

Minia Journal of Medical Research

Publication Link

https://mjmr.journals.ekb.eg/

MainTitle

The Possible Toxic Effects of Glutathione Intramuscular Injection on Ovaries of Female Albino Rats (Experimental Study)

Details

Type

Article

Created At

28 Dec 2024