Background: Pseudomonas aeruginosa (P. aeruginosa) infection shows a higher morbidity and mortality rates especially in immunosupperessed individuals. It has a high level of resistance through various mechanisms to most antimicrobials .Intrinsic, acquired, and adaptive resistance are the three types of resistance that P. aeruginosa develops to resist antibiotic .MDR P.aeruginosa is important cause of nosocombial infections Methods: From February 2022 to May 2023, 48 samples collected from various infected surgical wound specimens were used in this work. Then, antimicrobial susceptibility tests were done to the isolates by the disc diffusion method .PCR used for detection of gene responsible for antibiotic resistance. Results: Out of 48 samples, 25 isolates were positive for P. aeruginosa and (18/25) 72% were MDR. Conclusion: P. aeruginosa Infections have become a severe problem that requires a precise diagnosis and effective treatment. To replace the current unsuccessful therapies, new alternative treatment plans should be taken into consideration and researched.