Background: Episiotomy is the procedure that help to promote difficult vaginal deliveries to control and decrease dangerous perineal lacerations during normal labour.  The aim of this study was to evaluate the effect of local injection of Autologous Platelet-Rich Plasma (PRP) Solution in healing of episiotomy wounds. Methods:  Randomized controlled trial included 200 patients undergoing their first childbirth at Minia Maternity University Hospital and El-Fashn General Hospital between May 2021 and December 2021 randomized into either PRP (study) or control groups. In the study group, PRP solution was injected in subcutaneous tissue of wound while wounds were only cleaned with saline in the control group .Wound healing and pain were assessed using REEDA, VAS and VSS scores. Results: REEDA score was significantly lower in the PRP group compared to the control group at one, two and four weeks (1.5±0.49 vs. 1.85±0.7, 1.19±0.46 Vs. 1.65±0.5 and 1.65±0.5 vs. 1.65±0.5, respectively P=0.0001). Similar results were shown regarding VSS score in favour of PRP (1.13±0.57 vs. 1.62±0.75, 1.1±0.52 vs. 1.48±0.65 and 0.5±0.34 vs. 1.1±0.58, respectively, P =0.0001). Lower pain scores were achieved in the PRP group as compared to the control group; as evidenced by Vas scores (3.22±0.78 vs. 3.09±1.06, 2.18±0.49 vs. 2.45±0.66 and0.73±0.42 vs. 1.5±0.64, respectively, P = 0.001). Conclusion: PRP injection in episiotomy wounds is safe and may have a beneficial effect on wound healing and pain after vaginal delivery.