Background: Background: MS (multiple sclerosis) is an inflammatory demyelinating disease of the central nervous system. In spite of description of the grey matter involvement in MS, the conventional MRI sequences don't have the ability of detection of MS cortical lesions. Aim of Study: To assess the diagnostic value of DIR sequence in the detection of MS lesions by comparing DIR with FLAIR and T2-weighted pulse sequences in the brain. Methods: This Cross Sectional Study was carried out in Radio-diagnosis Department, Faculty of Medicine Minia University. 30 MS patients aged (20 to 50) years were included. MR imaging was performed to all patients (T1WI, T2WI, FLAIR, DIR, DWI and ADC sequences are performed in axial planes in addition to sagittal planes for FLAIR and DIR). Results: A total of 30 patients were enrolled in this study, females 26 (86.7%) predominated males 4 (13.3%). The median number of cortical and juxtacortical lesions ± IQR detected by DIR sequence (3.00 ± 3.00) and (2.00 ± 2.00) respectively was significantly higher than that detected by FLAIR sequence (1.00± 2.00) and (1.00± 1.00) respectively and T2 sequence (0.00 ±1.00) and (1.00± 2.00) respectively, with (P-value<0.0001 & <0.0001) respectively. DIR identified significantly more infratentorial lesions in comparison to FLAIR (P-value <0.039), but detected lower number of lesions when compared to T2 sequence, but the difference was below the statistical importance (p-value<0.275). Conclusion: DIR brain imaging sequence had the highest sensitivity in the detection of cortical and juxtacortical lesions compared with FLAIR and T2 sequences.