The using of chemical nematicides against the root-knot nematodes (Meloidogyne spp.) infections is the only famous route to most farmers in Egypt.  Root-knot nematodes are one of the destructive pests around the world. Moreover, biological agents as microbial pesticides are eco-friendly, effective and alternative tools to synthetic pesticides globally. In the current study, single and integration impacts of the nematicides fluopyram and oxamyl as well as Purpureocillium lilacinum against the root-knot nematode, Meloidogyne incognita on tomato plants in two separated pot experiments. The recorded reduction parameters of M. incognita were J₂/250g soil, galls and egg masses / 5g roots. The total dry weight, root and shoot lengths of tomato plants were also noticed. The total soluble protein /g fresh weight of tomato leaves was also recorded. The single applied treatments recorded general mean reduction (GMR) in soil population density at range of 70.33 to 85.24%, root galling recorded GMR at range of 57.62 to 77.35% and egg masses recorded GMR at range of 63.34 to 79.67%. However, the integrated treatments (in sequence) elucidated significant reductions in soil populations (from 72.22 to 88.85%), root galling (from 60.07 to 74.96%) and egg masses (from 62.81 to 81.90%). The applied treatments significantly increased the majority of measured plant indices, as well as exhibited fluctuation in the level of total soluble proteins (TSP) in both trials.