Eggplant crop (Solanum melongena L.) is one of the top ten vegetable crops grown in the world. It can be cultivated in different seasons during the year. Eggplants are known to be extremely susceptible to root-knot nematodes infection, causing severe damage leads to great losses. In the present study six eggplant varieties i.e., SNO-8-1, 108-3-1, BPCL-1, SCR-2, CLA-11-2 and SWD-13-1 were evaluated for their susceptibility to Meloidogyne spp. under greenhouse conditions. Results revealed that all tested varieties were susceptible to Meloidogyne spp. Variety (108-3-1) is considered the most susceptible one. Nematode parameters i.e., number of galls, egg masses, females/root system and J₂/250g soil were recorded (594, 381, 421 and 802) respectively. On the other hand, results showed that variety (BPCL-1) was the most tolerant one. In respect to the accumulation of total phenol compounds, HPLC was used to study the difference of chemical analysis between tolerant and susceptible eggplant varieties. Chromatogram analysis revealed that the highest total phenol concentration (36720.10 mAu) was detected in soil of root exudates of most tolerant variety (BPCL-1), in contrary the lowest accumulation of total phenol (32040.70 mAu) was recorded in soil of root exudates of susceptible variety (108-3-1). The genetic variation among the six eggplant varieties was studied too using the SSR technique, conducted with 10 different primers. The results showed genetic polymorphism among the six tested varieties, especially the EEMS15 and EEMS16 primers within the used primers.