Ciprofloxacin, a fluoroquinolone antibiotic, is commonly used to treat bacterial infections in humans owing to its broad antibacterial range. On the other hand, an overdose or excessive usage of ciprofloxacin may have a number of unfavorable consequences, not only on people but also on microbes. These side effects can be caused by ciprofloxacin. Using luminescence, an investigation was conducted to look at the interaction that occurs between ciprofloxacin and the Eu(III)-coumarin-3-carboxylic acid (Lc) complex. The changes in luminescence of the complex in solution were used as the basis for this inquiry. This investigation was based on the luminescence variations of the complex in solution in the absence and presence of ciprofloxacin. The effect of solvents on the interaction between the complex and antibiotic was investigated. The information obtained in this paper will be used in the development of new simple and efficient detection method of ciprofloxacin based on luminescent Eu(III)-complex as a chemosensor.