The present research goals to design a regenerative, motor, and rehabilitative treatment program in order to identify its effect on the injury of the anterior cartilage cut after regenerative treatment (prolotherapy) by studying the following objectives: Reducing pain, improving the muscle strength of the Agonists muscles, improving the range of motion of the knee joint after regenerative therapy. The author used an experimental procedure of one single group strategy with pre- and post- treatment evaluation. Participant (n= 8) have been recruited individuals who suffered from a cut in the medial anterior cartilage were injected with prolotherapy with ages between 50-70 years. They were all diagnosed with anterior cartilage cut after regenerative in the knee according to medical examination. Results specified that: commended rehabilitation exercises program exhibit encouraging impact on the working muscles and functional effectiveness of the injured knee joint during post- medical treatments. The pre- and post- treatment revealed statistically significant differences. post treatment's measurements revealed clear improvement in the thigh circumferences, muscle strength, pain degree, and range of motion effusion for the injured knee joint.