Background: In highly competitive world healthcare organizations must innovate, provide new services, and adopt innovative applications and new technologies in order to first survive and then grow. To maintain these roles, healthcare organizations need modern leadership styles that have an impact on supporting the characteristics of a learning organization that empowers nurses within and outside the organization to learn as they work and use technology to improve both learning and productivity where creating a supportive learning environment is an essential requirement for building learning. Aim: Explore leadership styles of first-line nurse managers and their relationship with characteristics of learning organization from nurses' perspective. Study design: This cross-sectional descriptive correlation study used to conduct. Setting was conducted in all inpatient units at four different hospitals affiliated to the Ministry of Health. Subject: A non-probability quota sampling technique was used to draw the study subjects from the entire population with the inclusion criteria of; a bedside nurse working in a technical position; having completed at least one year experience of service in the selected units, available at the time of data collection and be willing to participate in the current study (N=228)Tool Two tools were used in the current study. Tool I, The Learning Organization Assessment Survey The second tool I, Multifactor Leadership Questionnaire. Method The Two tools will be translated into Arabic, and back translation will be done Data were collected using self-administered questionnaires, which were hand-delivered to the study subjects at their working settings after explaining the study aim. Result: Statistical significant correlation was revealed between the multifactor leadership questionnaire and the learning organization assessment, Openness to New Ideas show coefficient (-0.212**)Overall supportive learning environment coefficient(-0.227**)statistically significant, (p=<0.001**), a positive statistically significant correlation was found between appreciation of differences (p=<0.001**), openness to new idea (p=0.001**), overall supportive learning environment (p=0.001**), education and training (p=<0.001**)and information transfer (p=0.026**). Conclusion: : Both transformational and transactional leadership styles impact organizational learning. Recommendation: Establish transactional leadership traits and transformational leadership characteristics as hiring criteria for first-line nurse managers, conduct an organizational-wide Learning Organization Assessment, and determine deficiencies in learning organization leadership training