Background: Microteaching is an organized, scaled-down teaching training strategy. That provides an opportunity to practice teaching skills by breaking them into smaller parts. Parallel to this novelty microteaching practice for nursing students, it requires them to be more responsible and independent in their learning process, considering cognitive, motivational, and behavioral variables of self-regulation to practice these skills effectively. Aim: To determine the effect of microteaching strategy on nursing students' self-regulation and teaching skills. Design: pre-experimental one group pre-test post-test research design. Settings: this study was carried out in the Faculty of Nursing, Alexandria University, Medical Surgical Nursing department. The application was done in faculty classes, medical surgical nursing labs, and information technology (IT) labs. Subjects: It included 132 nursing students who represented all nursing students enrolled in the "Medical Surgical Nursing III Course" during the first semester of the academic year 2022-2023. Tools: Two tools were used. Tool one: “Self-Regulation Assessment Questionnaire ". Tool two: “Students' teaching Skills Assessment Checklist". Results: The study showed that there was a significant improvement in students' self-regulation level after the application of microteaching program than before (P<0.001*). Furthermore, significant improvement in students' teaching skills level was found after the application of the strategy than before it(P<0.001*). Conclusion: Microteaching played an important role in enhancing the students' teaching skills, which include planning the lesson, presenting it, managing classroom and handling difficulties. Also, it helped students regulate their learning by encouraging them to set learning goals, use different strategies for achieving it through self-efficacy, interaction with their colleges, teachers, and evaluate to what extent these goals where achieved. Recommendations: Educational workshops should be conducted for all nurse educators to raise their awareness about microteaching when preparing their curriculum.