Background: In the Nursing workplace, incivility (WPI) is vital because a patient's quality of care may be adversely affected, directly or indirectly, and also reasons for nurses to leave or plan to leave the profession. Peers at the workplace are just an informal relationship that can influence, motivate, and/or control individuals more than what superiors intend to do, this peer relationship (PR) can help Nurses deal with WPI and conflicts when begin to arise. This study aimed to assess the relationship between WPI and PR as perceived by nurses. Setting: This study was conducted in all Medical Care Units and its specialties include 24 units Surgical Care Units and its specialties include 15 units at Alexandria Main University Hospital. Subjects: out of 343 nurses who provide direct and indirect care to patients and don't occupy any managerial positions were included in the study, they distributed as follows; 145 in medical units and 198 in surgical units. Tools: two tools were used to collect the data; the first tool was The Nursing Incivility Scale (NIS),.the second tool Peer relationship scale. Results: there was a statistically significant positive correlation between all dimensions of WPI and sources of incivility e.g. (general incivility, nurse Incivility, supervisor, physician, and patient) and total WPI.Additionally, the results showed negative correlations between WPI and its dimensions with PR. Conclusion: the present study concluded that the nurses perceived a moderate level of WPI, and there was a highly statistically significant negative correlation between WPI dimensions and PR. Recommendation: Create and disseminate a clear policy for WPI prevention and ensure that managers, supervisors, coworkers, patients, and visitors know about this policy promote civil dialogue as the norm and keep hospital standards recognized and followed by encouraging the WPI Zero-tolerance policy, develop effective communication between nurses and manager, in conjunction with good interpersonal relationships and social interaction are considered indispensable conditions for feeling comfortable with one's work.