Background: Healthcare Waste Management (HCWM) process enable to managed hemodialysis waste responsibly, without harming the personal, institution and the community. Hemodialysis wastes are risky for all people working in hemodialysis (HD) unit and exposed to waste as well as the community. Aim of this study: The study aims to assess HCWM process in Hemodialysis Unit in El Beheira governorate. Research design: A descriptive cross sectional research design was used to carry out this study. Settings: The study was carried out in five HD units located within five general hospitals allocated to four health directorates in El Beheira governorate. Subjects: All nurses and cleaning workers who working in the previously mentioned set-tings. Data collection tools: The following four tools were used in order to collect the required data from the study sample; Tool I: Nurse's and Cleaning Worker's Socio-Demographic Characteristics, Health Profile and Occupational Data A Self-administered Structured Questionnaire Sheet for the Nurses and A Structured In-terview Schedule for the Cleaning Worker's. Tool II: Knowledge of Nurses and Cleaning Workers about HCWM Process in HD Unit A Self-administered Structured Questionnaire Sheet for the Nurses and A Struc-tured Interview Schedule for the Cleaning Worker's. Tool III: Practice of Nurses about HCWM Process in HD Unit Structured Observation Checklist. Tool IV: Practice of Cleaning Workers about HCWM Process in HD Unit Structured Observation Checklist. Results: The highest percent (94%) of the studied nurses and more than three quarters (85.7%) of the studied cleaning workers had good level of knowledge. The highest percent (88.6%, 81%) of the studied nurses and cleaning workers had good level of practice respectively. Con-clusion: The highest percent of the studied nurses and cleaning workers had good level of knowledge and prac-tice about healthcare waste management pro-cess in HD unit in El- Beheira Governorate. Recommenda-tions: HD unit should have a close monitoring and supervision to ensure the application of safety regulations in waste handling and develop effective implementations of HCWM plan