Background: .The prevalence of urinary tract infections (UTIs), globally, is a major type and most common of HAIs and represent around 40% of HAIs and also, one of the top ranking microbial infections .Urinary tract infections(UTIs) associated with catheters threats patient's safety worldwide, it is infection called Catheter Associated Urinary Tract Infection(CAUTI). Objective: To assess urosurgical nurses' adherence to infection prevention and control practices Settings: The study was carried out in the inpatient Urosurgical Ward of the Main University Hospital at Alexandria, the inpatient Urosurgical Wards of Gamal Abd El Nasser Health Insurance Hospital at Alexandria, the inpatient General Surgical Ward of Shark El Madena Hospital at Alexandria and the inpatient Urosurgical Wards of the National Medical Institute, Damanhour, El Beheira Governorate. Egypt. Subjects: All nurses were worked at the above-mentioned settings who were available at the time of data collection: the Main University Hospital: around 14 nurses, Gamal Abd El Nasser Health Insurance Hospital: approximately 7 nurses, Shark El Madena Hospital: around 20 nurses, The National Medical Institute, Damanhour: 9 nurses. Tools: two tools were used. Tool one: “Urosurgical Nurses' Adherence with Infection Prevention and Control Practices: An Observation Checklist". Tool two: “Urosurgical Nurses' Adherence to Infection Prevention and Control Practices (IPCP):A Questionnaire of Knowledge". Results: The study showed that statistically significant differences, were detected among the four hospitals “safe injection practices" where (p=0.027). No statistical significant differences, were detected among the studied four hospitals, nurses regarding other nurses' practices, as well as the “overall nurses' practice", as determined different “P" values there was a statistical significant relationships were found between the nurses' overall knowledge and the nurses' gender and attendance of training courses on infection prevention and control practices (p=0.002, p=0.042) respectively. Conclusion: More than half of the studied nurses (56%) had “moderate" knowledge and only (2%) of them had “good knowledge" and the overall nurses' adherence to practice standards, was “poor" and (100%) had “poor" adherence. Recommendations: Continuing in-service training sessions are programs for nurses, about IPCPs, are required and monitoring and feedback of nurses' adherence should be periodically, reported.