To study the wheat productivity response to some bio-fertilizer types and nano silica rates under the New Valley conditions, a field experiment was implemented out in a private farm at the two successive growing seasons (2021/2022 and 2022/2023) in the New Valley governorate (El-Dakhla Oasis). The experiment was laid out using randomized complete blocks design in split plot arrangement were, the biofertilizers (Cerialen, Rhizobacteria and Azotobacter) plus control were assigned to the main plots, and Nano-Silica concentrations (0, 200, 400 and 600 ppm) were allocated in the sub-plots with four replications. All studied traits affected significantly by bio-fertilizer and nano silica rates in both seasons. Where, the Rhizobacteria biofertilizer gave tallest plants, longest spikes, the highest spikes number/m2, the heaviest weights of 1000-grain and grain yield/fad. Here too, increasing nano silica concentrations up to 600 ppm raise the number of spikes/m2, weight of 1000-grain and grain yield/fad, as well as plant height and spike length in both seasons. Furthermore, biofertilizers x nano-silica concentration interaction exhibited a significant impact on all studied traits in both seasons, except grain yield/fad in the first season. Rhizobacteria x 600 ppm interaction gave the highest mean values of plant height, spike length, number of spikes/m2, 1000 grain weight and grain yield/fad in both seasons.