This investigation was carried out at Faculty of Agriculture Farm New Valley University (EL-Kharga), during two experimental seasons of 2021/2022 and 2022/2023  to explore the impact of the addition of humic acid at rats 100, 200, and 300 ml/l. plus, half of the recommended dose of NPK fertilizer and bio-fertilization (mycorrhizae and azolla), on plant growth, yield, components, and essential oil and chemical constituents of caraway (Carum carvi L.)plants. Results showed a significant increase in plant growth traits in terms of plant height, branch number, plant fresh and dry weight, yield i.e., umbel number per plant fruit yield per plant and feddan, as well as volatile oil production. Treating plants with humic acid at higher concentration of 300 ml/l recorded the highest values of these studied parameters. Also, adding mycorrhizae fungi (MY) with azolla extract together effectively increased the traits under this study. All tide coefficients were significantly affected by the interaction coefficients. In this regard, most combined treatments significantly increased all traits under study. Then humic acid at a high concentration (300 ml/l) plus inoculation caraway plants with mixed mycorrhizae fungi and azola were the most effective treatments. The main constituents of volatile oil were also affected by humic acid and biofertilizer applications. The results of gas chromatographic analysis (GC/MS) of caraway oil revealed 15 compounds. When comparing the values of the chemical compounds of the volatile oil, it is noticed that the Carvone-D-Limonene-cis-Anethole compounds contain the highest percentages of volatile oil compounds compared to the other compounds.