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The Effect of Ethanolic Extract of Stinging Nettle (Urtica Dioica) on the Larval Stages of Greater Wax Moth (Galleria mellonella) Lepidoptera

Article

Last updated: 28 Dec 2024

Subjects

-

Tags

Plant protection and plant diseases

Abstract

The present study was carried out at the Duhok University College Laboratory Centre in the Agricultural Engineering Sciences from September 2021 to June 2022, to evaluate the toxic effect of stinging nettle ethanolic extracts of stinging nettle (Urtica dioica) at different concentrations on the larval stages of the Greater wax moth (Galleria mellonella, Lepidoptera: Pyralidae). The Soxhlet extraction method was used in this study, with five concentrations (0.5, 1, 1.5, 2.5, and 3.5 mg/ml) of the ethanolic plant extract after 24, 48, and 72 hours of exposure. The third and fifth instar larvae of the insect were examined under the conditions (at 30±1°C and 70±5% R.H). There was a highly significant influence (P < 0.001) on concentrations, affected mortality statistically significant (P < 0.05). The results of the stinging nettle extracted at 3.5 mg/ml after 72 hours of exposure were obtained and the highest mortality rate recorded in the 3rd and 5th instar larvae which were 96.66 and 86.66% respectively. The ethanolic Nettle Stinging extract was testified to cause morphological deformations in both larval instars of the insect.

DOI

10.21608/nvjas.2023.212480.1213

Keywords

Urtica dioica, Galleria mellonella Extraction method, mortality rate

Authors

First Name

Badia

Last Name

Al-Chalabi

MiddleName

Mahmoud

Affiliation

Department of Biology. Faculty of Science, University of Zakho, Iraq.

Email

badeyamahmoud50@gmail.com

City

-

Orcid

-

First Name

Sofyan

Last Name

Al-Sinjari

MiddleName

Sedo

Affiliation

Department of Biology. Faculty of Science, University of Zakho, Iraq.

Email

sofyan.sedo@uoz.edu.krd

City

-

Orcid

-

First Name

Mehvan

Last Name

Rasheed

MiddleName

Ali

Affiliation

Department of Biology. Faculty of Science, University of Zakho, Iraq.

Email

mehvanqassari@gmail.com

City

-

Orcid

-

Volume

3

Article Issue

8

Related Issue

40586

Issue Date

2023-04-01

Receive Date

2023-05-21

Publish Date

2023-06-23

Page Start

869

Page End

876

Print ISSN

2805-2420

Online ISSN

2805-2439

Link

https://nvjas.journals.ekb.eg/article_304896.html

Detail API

https://nvjas.journals.ekb.eg/service?article_code=304896

Order

10

Type

Original Research

Type Code

2,101

Publication Type

Journal

Publication Title

New Valley Journal of Agricultural Science

Publication Link

https://nvjas.journals.ekb.eg/

MainTitle

The Effect of Ethanolic Extract of Stinging Nettle (Urtica Dioica) on the Larval Stages of Greater Wax Moth (Galleria mellonella) Lepidoptera

Details

Type

Article

Created At

28 Dec 2024