Background: Talent management has been on the agenda of many health care organizations because of the belief in its importance in achieving organizational excellence and makes their personnel more satisfied to achieve organizational objectives. Aim of this research: To investigate the effectiveness of educational program to improve first line nursing managers' knowledge and practices regarding talent management at main Assiut University Hospital. Research design: A quasi-experimental design was utilized in this research. Setting: This research conducted at Main Assiut University Hospital. Sample: The study subjects consisted of all first line nursing managers who worked at the Main Assiut University Hospital during the period of data collection with total number (80) first line nursing managers. Tools of data collection: Two tools as follow, the first tool was talent management knowledge questionnaire and the second tool was talent management practice questionnaire. Results: there was high statistically significant improvement in the first line nursing managers' level of talent management knowledge and practice after intervention both immediately post and follow up program (p=.001** & p=.002**) respectively. Conclusion: there were improvement in the first line nursing managers' level of talent management knowledge and practice after the educational program. Recommendations:  Conduct talent management workshops periodically for all nursing managers at all levels based on their job description as a refreshment course.