Background: Worldwide, work stress among care professionals, nurses in particular, is a major concern in health care systems; it is linked to high rates of job dissatisfaction, burnout, absenteeism, turnover, and stress-related illness, thus placing job performance among nurses' and patients' lives at risk.  Aim of the study: is to assess Work Stress among Nursing Staff and its association to their Organizational Social relations. Research design: a descriptive correlational research design. Setting: conducted at the Minia Health Insurance Hospital. Subjects: all nursing staff at the time of data collection (no=249). Data collection tools: data was collected by using two tools; as self-administered questionnaire consisted of part one socio-demographic data and part two work stress, tool two was organizational socialization. Results: the study showed that more than two-thirds of nursing staff have total moderate level of work stress and more than one quarter had high level; also, more than half of nursing staff have high score level of total organizational socialization, and less than half of nursing staff have moderate level score of  organizational socialization. Conclusion: there was a negative weak association between work stress and organizational social relations among nurses. Recommendation: nurses should have training session regarding coping strategies with stress and support each other in their work to decrease their stress.