Background: Postnatal care is one of the most important maternal health cares for prevention of maternal impairment and disabilities. So, there is an urgent need to increase women's awareness regarding self-care measures during postpartum period. Aim: This research aimed to evaluate the effect of self-care guidelines on women awareness regarding post-partum perineal wound care. Research design: A quasi-experimental research design was used in this research. Sample: Purposive sample include 70 post-partum women according to the G power formula. Setting: Minya University Hospital for Obstetric and Pediatric (MUHOP) at the postpartum word. Tools: Two tools used in this research. First tool: Interview questionnaire include socio-demographic characteristic, Second tool: Perineal wound care knowledge questionnaire. Results: The finding of the study illustrated that (5.5 %) of studied women have good knowledge about perineal care on pre-self-care guidelines compare with (85%) of them on post self-care guidelines. Conclusion: There were a marked improvement in women's total knowledge as well as all items of the knowledge regarding post-partum perineal wound care on the post self care guidelines than pre self care guidelines with statistically significant difference. Recommendation: Self-care guidelines about women awareness regarding post-partum perineal wound care should printed and distributing to women who having perineal wound.