Background: Cleft lip and palate (CLP) is one of the most common congenital craniofacial abnormalities in the world, genetic and environmental factors play a significant role in its etiology. Pre-operative nursing education to mothers of cleft palate infants is directed toward improving infants feeding. Aim of the study: The aim of the current study was to evaluate the impact of an educational program regarding cleft palate on mother's awareness and their infants feeding pattern. Research design: Quasi-experimental research design (pre-posttest) was used in the current study. Setting: The current study was conducted at out-patient surgery unit at Minia university hospital for obstetric and pediatric, which located in Minia city. Subjects: A purposeful sample of 60 mothers of children with cleft palate was participated in the study: 30 for study group and 30 for control group. Research tools: Tool (I): A structured interview questionnaire: it covered sociodemographic data of mothers and data of infants and mothers' knowledge about cleft palate; Tool (II): Feeding pattern mothers' practice assessment checklist; Tool (III): Observational checklist about bottle feeding preparation; Tool (IV): Egyptian Growth Curve Chart (EGCC). Results: It was found that there were highly statistically significant differences between the study and control groups of mothers' knowledge regarding cleft palate in pre, post and follow up-test P-value at 0.000001. As well as almost of the mothers in the post-test in the study group had appropriate level of practice in regarding feeding infants with cleft palate. The results of the current study showed that there is a positive correlation between the total score of mothers' knowledge and their practices in pre and post-test, and follow up test Conclusion: The study concluded that the preoperative instructions for mothers of cleft palate infants were effective in the improvement of mothers knowledge, practices and infant weight and feeding pattern .Recommendation: The study recommended that developing continuous training session to pediatric surgical nurses and educational sessions for mothers about cleft palate and feeding infants with cleft palate would improve mothers' knowledge, practices and their infants feeding pattern.