Healthy infants at weaning age are growing and developing very fast, so great care must be taken to ensure they are getting enough of the right food. The aim was to investigate the effect of health guidelines on mothers' awareness regarding weaning practices. Design: A quazi –experimental design was used. Settings: The present study was collected from Suzan medical center and the general medical center of Alfekria city. Tools: three main tools was used: A first was Socio-demographic parts of the newly lactated mothers, the second was the problem and challenges of weaning, the third was mother's awareness related to weaning, which consisted of three subparts including mother's knowledge, attitude, and weaning practices. Results: Nearly half of the newly lactated mothers have secondary education, and more than half are aged between 25 and 29. More than two-fifth of the newly lactated mothers with good knowledge levels before implementing health guidelines improved to almost them after implementing health guidelines. The vast majority of the newly lactated mothers who had a positive attitude before implementing health guidelines turned to the most after implementing health guidelines. Also, the majority of the participant mothers had good practices before implementing health guidelines and improved most of them after implementing health guidelines. Conclusion: Newly lactated mothers' awareness was improved after implementing health guidelines about weaning, which indicated the effectiveness of the used guidelines on improving their knowledge, attitude, and practices. Recommendations: continues health educational guidelines for newly pregnant women to prepare them for future responsibility regarding their health and their infants.